エンドトキシン投与時におけるラット肝病変の電子顕微鏡的研究

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  • An electron microscopic study on the rat liver following endotoxin administration
  • エンドトキシン トウヨジ ニ オケル ラット カン ビョウヘン ノ デンシ ケ

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The ultrastructural changes produced in the rat liver following intraperitoneal injection of a single close of endotoxin were studied for 24 hours. Perfusion fixation was used for suitable preservation of the ultrastructure of the liver, especially that of the sinusoids. During the first 3 hours after the administration of endotoxin, hepatocytes showed a loss of glycogen accompanied by an increased prominence of smooth endoplasmic reticulum, swelling of mitochondria, disorganization of rough endoplasmic reticulum and an increase in numbers of lysosomes and lipid droplets. These changes were similar to those seen in saline injected animals and considered to be nonspecific alterations elicited by stress. In the sinusoids there was an aggregation of leukocytes, platelets and erythrocytes at 1 hour. In addition, at later intervals a considerable amount of fibrin was found. At 4 hours hepatocytes showed a marked vacuolation and these vacuoles were bounded by a single membrane and occasionally contained fibrillar materials. Necroses of hepatocytes were seen in the periportal area. In the sinusoids there were large numbers of thrombi. Endothelial cells around the thrombi showed desquamation and fragmentation and the space of Disse was markedly distended with appearance of erythrocytes. By 24 hours these changes appeared less prominent. The characteristic change in hepatocytes at this interval was an increase in numbers of very low density lipoprotein granules in the Golgi vesicles, endoplasmic reticulum and Disse's space and was thought to he a finding indicating recovery from the inhibition of protein synthesis. It is concluded that thrombi formation by endotoxin plays an important role in the initation of hepatocellular necrosis.

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