Thin layer chromatography of urea compounds

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  • 尿素化合物の薄層クロマトグラフィー
  • ニョウソ カゴウブツ ノ ハクソウ クロマトグラフィー

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Thin layer chromatography of urea compounds was investigated comparative to paper chromatography using silica gel G. The compounds were detected as yellow spots by spraying Ehrlich's reagent followed by heating at 110℃ for 10 minutes. Butyl alcohol : acetic acid : water (3:1:1), phenol : water (3:1), iso-propyl alcohol : formic acid : water (7:1:2) and amyl alcohol saturated with water were examined as solvent. Using these solvents, Rf values of urea, biuret, monophenylurea, ethyleneurea, nonamethylurea, isobutylindendiurea and uraform were examined by one-dimensional ascending procedure. Good separation of these compounds was obtained. Among the solvents, it was found that butyl alcohol: acetic acid: water is the best and amyl alcohol saturated with water is the next. On the sample of uraform, 3 spots were detected by the former and 2 by the latter, although each one spot was estimated by others. Clear yellow spot of urea was detected with butyl alcohol : acetic acid : water as solvent when 1 fig of urea had been applied. Vague one was also observed with 0.1 to 0.5 μg, contrary to 5 μg on paper chromatogram. As practice, reaction products from ^14C-urea by urea dehydrogenase of perilla leaf were separated by thin layer and paper chromatographies. ^14C-urea was treated with some kinds of reagents such as p-dimethylaminobenzaldehyde, ninhydrin, sodium nitrite, potassium ferricyanate, sodium nitroprusside, potassium permanganate and iodine. These reaction mixtures were chromatographed on thin layer and paper too. Radioactive compounds were detected by autoradiography. In these cases, better results were obtained by thin layer chromatography.

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