Production of Oyster Extract by Holizontal Rotary Column Reactor

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  • 横型回転カラム式リアクターによるオイスターエキスの製造
  • ヨコガタ カイテン カラムシキ リアクター ニヨル オイスターエキス ノ セイ

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Abstract

Continuous hydrolysis of protein with an immobilized protease was performed by using a holizontal rotary column reactor for the continuous production of oyster extract. Porous silica beads were used as a support for immobilization of enzyme and Denazyme AP for food industry use was used as enzyme protease. Activation method with glutaraldehyde (GA) were applied for the covalent immobilization of enzyme and the condition of each step for immobilization was investigated in detail. On searching the immobilization conditions, pH 3 and pH 8 were chosen as the GA activation pHs considering not only the differences of the chemical state of GA in acidic and alkaline media but also those of manner of binding: As the results, the optimal conditions were appeared to be 0.5% of GA concentration, 6 hrs of GA activation time and 27500U/ml of enzyme concentration in the case of pH 3. At pH 8, the best conditions were 0.15% (GA concentration), 2 hrs (GA activation time) and 22000U/ml (enzyme concentration). GA activation at pH3 was chosen because the immobilized supports at pH3 gave higher activity than those at pH8. The best coupling time of the activated supports with enzyme was 16 hrs. The immobilized supports produced with the optimal conditions were filled in the holizontal rotary column reactor and the continuous hydrolysis of oyster paste (2.5 times diluted material) was performed at 50℃. Oyster extract with good quality was produced at the conditions of 20 rpm (rotational frequency of drum), 50% (filling ratio of supports), 50% (filling ratio of oyster paste) and 2.5 hrs (residence time). The production of oyster extract was continued for 24 hrs under an aseptic condition.

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