Development of Automatic Immunosensor System for Quantification of Allergens
-
- Higuchi Motonobu
- Institute of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University
-
- Kuwano Takahisa
- Institute of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University
-
- Matsumoto Kiyoshi
- Institute of Food Biotechnology, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University
Bibliographic Information
- Other Title
-
- アレルゲン自動計測用免疫センサーシステムの開発
- アレルゲン ジドウ ケイソクヨウ メンエキ センサー システム ノ カイハツ
Search this article
Description
A glassy carbon electrode, anodized for a short time at about 2.0 V vs. Pt,is a very convenient regenerable solid phase for the successive steps of an automated enzymelinked immunosorbent assay. The anodized surface absorbs allergen and the blocking proteins in a similar manner to classical polystyrene solid phase; the detection of the immobilized alkaline phosphatase used as a label can be achieved from an enzymatic process occurring; and the protein stacking, accumlated during the assay, can be eliminated by a simple re-anodization at the end of the cycle, allowing the adsorption of a new allergen. Using peroxidase or alkaline phosphatase as labels, various allergens such as ovalbumin, ovomucoid, and β-lactoglobulin were assayed.
Journal
-
- 九州大學農學部學藝雜誌
-
九州大學農學部學藝雜誌 54 (1/2), 7-16, 1999-11
Faculty of Agriculture, Kyushu University
- Tweet
Details 詳細情報について
-
- CRID
- 1390009224837934336
-
- NII Article ID
- 110000156510
-
- NII Book ID
- AN0005519X
-
- ISSN
- 03686264
-
- DOI
- 10.15017/23628
-
- HANDLE
- 2324/23628
-
- NDL BIB ID
- 4926548
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- IRDB
- NDL
- CiNii Articles