2,2ʼ,4,4ʼ,6,6ʼ-六塩素化ビフェニル(PCB155)のラット, モルモットおよびヒト肝ミクロゾーム, およびヒトチトクロムP450による代謝
書誌事項
- タイトル別名
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- Metabolism of 2,2ʼ,4,4ʼ,6,6ʼ-Hexachlorobiphenyl (PCB155) by Rat, Guinea Pig and Human Liver Microsomes and Human Cytochrome P450s
- 2,2'4,4'6,6'-六塩素化ビフェニル(PCB155)のラット,モルモットおよびヒト肝ミクロゾーム,およびヒトチトクロムP450による代謝
- 2,2'4,4'6,6'-ロク エンソカ ビフェニル(PCB155)ノ ラット,モルモット オヨビ ヒト カン ミクロゾーム,オヨビ ヒトチトクロム P450 ニ ヨル タイシャ
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The in vitro metabolism of a 2,4,6-trichloro-substituted PCB, 2,2ʼ,4,4ʼ,6,6ʼ-hexachlorobiphenyl (PCB155), was studied using rat, guinea pig and human liver microsomes, and human cytochrome P450 (CYP) isoforms. Three kinds of liver microsomes from untreated, phenobarbital (PB) -treated and 3-methylcholanthrene (MC) -treated rats and guinea pigs were also used. In rats, PCB155 was metabolized to M1 by PB-microsomes with a remarkable high rate of 4.554 nmol/hr/mg protein, but not by both untreated microsomes and MC-microsomes. In guinea pigs, both untreated microsomes and MC-microsomes showed the M1-producing activity with rates of 0.056 and 0.060 nmol/hr/mg protein, respectively, and the activity was increased to3 .5-fold of untreated microsomes by PB treatment. GC-MS analysis demonstrated that the methylatedM1 had the molecular weight of 388 and almost completely agreed with a synthesized authentic 3-methoxy-PCB155 with regard to the retention times and mass fragmentation. Of four human CYP isoforms, only CYP2B6 showed high activity to form M1 at a rate of 0.702 nmol/hr/nmo l CYP. These results suggest that the major metabolite formed by PB-inducible CYP2B enzymes in rats, guinea pigs and human is 3-hydroxy-PCB155, and that CYP2B6 plays the most important role in PCB155 metabolism in human liver.
収録刊行物
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- 福岡醫學雜誌
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福岡醫學雜誌 112 (2), 127-135, 2021-06-25
福岡医学会
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詳細情報 詳細情報について
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- CRID
- 1390009225548359424
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- NII論文ID
- 120007124568
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- NII書誌ID
- AN00215478
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- DOI
- 10.15017/4483198
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- HANDLE
- 2324/4483198
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- NDL書誌ID
- 031734915
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- ISSN
- 0016254X
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用可