Study of Rapid HPLC Method for the Determination of Antioxidants in Foods, and Standard Solution Stability during Storage
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- Mikami Yoko
- Tokyo Metropolitan Institute of Public Health
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- Takagi Yuko
- Tokyo Metropolitan Institute of Public Health
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- Miyakawa Hiroyuki
- Tokyo Metropolitan Institute of Public Health
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- Yamajima Yukiko
- Tokyo Metropolitan Institute of Public Health
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- Sakamaki Narue
- Tokyo Metropolitan Institute of Public Health
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- Kobayashi Chigusa
- Tokyo Metropolitan Institute of Public Health
Bibliographic Information
- Other Title
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- 食品中酸化防止剤の迅速HPLC法および標準溶液の長期安定性の検討
- ショクヒン チュウ サンカ ボウシザイ ノ ジンソク HPLCホウ オヨビ ヒョウジュン ヨウエキ ノ チョウキ アンテイセイ ノ ケントウ
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Abstract
<p>In this study, we simultaneously determined three antioxidants, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and tert-butylhydroquinone (TBHQ), using HPLC equipped both with a photodiode array detector and a fluorescence detector in 25 minutes per sample. Due to the combined use of the two detectors, we could achieve improved target selectivity. Further, quantification at the specific wavelengths for each target substance particularly increased BHT detection sensitivity. This approach enabled us to avoid repeated measurements during daily inspections. Furthermore, detections were performed using LC-MS/MS instead of GC-MS to overcome the problem of helium gas shortage.</p><p>In addition, we investigated antioxidant stability in standard solutions during storage. Although TBHQ was stable in methanol with ascorbic acid at −20℃, ascorbic acid storage has possibility to lead to decrease in BHT and BHA concentrations. We recognized that the mixture of BHT and BHA dissolved in methanol at 4℃ and that of BHT, BHA and TBHQ dissolved in methanol with ascorbic acid at −20℃ were suitable for about one year.</p>
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 63 (1), 12-19, 2022-02-25
Japanese Society for Food Hygiene and Safety
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Keywords
Details 詳細情報について
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- CRID
- 1390010292591866496
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- NII Book ID
- AN00117741
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 032024652
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
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- Abstract License Flag
- Disallowed