Validation Study on an Improved Quantitative Method for Aflatoxins in Foods
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- Yoshimitsu Masato
- Osaka Institute of Public Health
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- Uchida Kotaro
- Osaka Institute of Public Health
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- Osakada Masakazu
- Osaka Institute of Public Health
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- Matsui Hiroshi
- Osaka Institute of Public Health
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- Ueno Ryo
- Osaka Institute of Public Health
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- Fujiwara Takuya
- Osaka Institute of Public Health
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- Akutsu Kazuhiko
- Osaka Institute of Public Health
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- Shinya Masanao
- Osaka Institute of Public Health
Bibliographic Information
- Other Title
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- 食品中のアフラトキシン定量分析における改良法の妥当性評価
- ショクヒン チュウ ノ アフラトキシン テイリョウ ブンセキ ニ オケル カイリョウホウ ノ ダトウセイ ヒョウカ
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Abstract
<p>As an analytical method for aflatoxins in foods, the analytical method based on the notification by the director of the Food Safety Department, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare (August 16, 2011) has been established. In order to improve the operability and analytical performance of the conventional method, this study aimed to construct an improved method that optimized selection of immunoaffinity column (IAC) and purifying condition, and omitted evaporation after the purification with IAC. In the recovery test performed by adding 2.5 ng/g of aflatoxin B1, B2, G1 and G2 standard solutions into 9 kinds of food samples, the improved method achieved the established target values: 77.0–99.7% of recovery, 1.7–5.6% of intra-assay coefficient of validation, and 0.9–3.6% of inter-assay of coefficient of variation, respectively. The improved method also achieved 4.3–10.5% greater recovery and 1.5 hours shorter preparation time than the conventional one. These results indicate applicability of the improved method for 9 kinds of foods and its efficacy as an analytical method for aflatoxins in foods.</p>
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 63 (1), 43-46, 2022-02-25
Japanese Society for Food Hygiene and Safety
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Details 詳細情報について
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- CRID
- 1390010292591869184
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- NII Book ID
- AN00117741
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 032024677
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
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- Abstract License Flag
- Disallowed