人工HDLによる骨格筋 筋芽細胞のミトコンドリア機能促進効果

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  • HDL mimetics promotes mitochondrial function in mouse myoblast cells.

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<p>High-density lipoprotein (HDL) has been reported to have pleiotropic effects for antiatherosclerosis, and large clinical trial has been shown that an induction of HDL by torcetrapib improves glucose metabolism. Recently, HDL and apolipoprotein A-I (ApoA-I), the major constituting protein of HDL had been demonstrated to enhance mitochondrial function in skeletal muscle in vitro. </p><p>One of the HDL mimetics, Fukuoka University ApoA-I Mimetic Peptide (FAMP) was developed as a low-amino acid residues peptide preserving human ApoA-I activity without phospholipids and has been reported to enhance HDL functions. In this study, we evaluated whether FAMP with HDL would affect mitochondrial functions in C2C12 mouse myoblast cells using with the extracellular flux analyzer. As the results, HDL induced oxygen consumption rate changes that was the significant elevation of basal respiration (+35%), maximal respiration (+54%), ATP production (+35%) and spare respiratory capacity (+68%). Furthermore, an incubation of HDL with FAMP at 37℃ significantly increased maximal respiration and spare respiratory capacity in a dose dependent manner.</p><p>In conclusion, HDL mimetics improved mitochondrial function in skeletal muscle cells through enhancement of HDL function.</p>

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