Effect of an Oil Medium on Giant Vesicles Prepared with Water-in-Oil Emulsion

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  • TOYOTA Taro
    Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo Universal Biology Institute, The University of Tokyo
  • ZHANG Yiting
    Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo

Bibliographic Information

Other Title
  • 油中水滴エマルションで調製するジャイアントベシクルに対する油性分散媒の影響

Abstract

<p>A closed lipid bilayer membrane with a diameter of one micrometer or more in water is called as giant vesicle and it is attracting attention as a cell mimicry reaction space because its structure and size are similar to those of living cells. When utilizing giant vesicles from a cell model perspective, biomolecules are needed to be encapsulated inside giant vesicles under conditions close to the cytoplasm. In recent years, the development of this encapsulation technology has been accelerating. The origin of this trend is a preparation method of giant unilamellar vesicles using a water-in-oil emulsion, published by a research group at Harvard University in 2003. The preparation method can only be performed on a desktop centrifuge. Here, we report on the experimental results of this preparation method using different oil for the water-in-oil emulsion.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 71 (1.2), 83-89, 2022-01-05

    The Japan Society for Analytical Chemistry

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