Nondestructive Preincubation Sex Determination of Broiler Eggs Using Visible Transmission Spectroscopy Combined with Multivariate Analysis

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  • RAHMAN Afzal
    Graduate School of Agriculture, Kyoto University Faculty of Agricultural Engineering and Technology, Bangladesh Agricultural University
  • SYDUZZAMAN Md
    Graduate School of Agriculture, Kyoto University Faculty of Applied Science and Technology, Jashore University of Science and Technology
  • KHALIDUZZAMAN Alin
    Graduate School of Agriculture, Kyoto University Faculty of Agricultural Engineering and Technology, Sylhet Agricultural University
  • FUJITANI Shinichi
    NABEL Co. Ltd.
  • KASHIMORI Ayuko
    NABEL Co. Ltd.
  • SUZUKI Tetsuhito
    Graduate School of Agriculture, Kyoto University
  • OGAWA Yuichi
    Graduate School of Agriculture, Kyoto University
  • KONDO Naoshi
    Graduate School of Agriculture, Kyoto University

Abstract

To confirm the spectral absorbance associated with differences in yolk ratio would provide a means to determine the sex of broiler eggs, we measured light transmission (200–955 nm) through ROSS 308 broiler eggs (n = 269) prior to incuba- tion. Statistical analysis demonstrated that male eggs had a significantly higher absorbance (500–654 nm) than female eggs. Similarly, eggs with a higher yolk ratio had significantly higher absorbance than eggs with a lower yolk ratio, sug- gesting that the yolk ratio plays an important role in sex-related spectral absorbance differences. Moreover, a supervised logistic regression classifier model based on these wavelengths determined the sex of eggs with an accuracy of 76 %.

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