Investigation of Practical Conditions for the Absorption and Transport of Soybean Oil (Rich in Long-Chain Triglycerides) and Medium-Chain Triglycerides Using Cellulose-Acetate Membrane Electrophoresis

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  • Yoshimura Ryoji
    Department of Health and Nutrition, Faculty of Health Management, Nagasaki International University
  • Nomura Shuichi
    Department of Health and Nutrition, Faculty of Health Management, Nagasaki International University

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Other Title
  • セルロースアセテート膜電気泳動法を用いた大豆油（長鎖脂肪酸油）と中鎖脂肪酸油の吸収，輸送経路に関する実習条件の検討
  • セルロースアセテートマク デンキ エイドウホウ オ モチイタ ダイズアブラ(チョウサ シボウサンユ)ト チュウ サ シボウサンユ ノ キュウシュウ,ユソウ ケイロ ニ カンスル ジッシュウ ジョウケン ノ ケントウ

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Abstract

<p>Objective: To understand lipid absorption and transport and the role of lipoproteins in these processes is crucial for planning dietary therapy, selecting therapeutic foods, and understanding the pathogenesis of dyslipidemia and atherosclerosis. Therefore, this study was conducted to determine the conditions for experiments done by the students aimed at understanding the processes involved in the absorption and transport of lipids and the functions of lipoproteins in these processes, using soybean oil, which contains long-chain fatty acids, and caprylic-capric triglyceride (CCT), which contains medium-chain fatty acids.</p><p>Methods: Seven-week-old male Wistar rats were fed a high-fat diet containing 45% fat by kilocalorie, and the equivalent amount of soybean oil intake (in ml) was calculated based on the food intake, diet composition, and specific gravity of soybean oil. The rats divided into the following three groups: tap water group, soybean oil group, CCT group. The rats were gastrointestinally administered tap water, soybean oil, or CCT at 1 ml/100 g body weight. Blood was collected three hours after administration of the individual treatments, following which the plasma triglyceride levels were measured; lipoprotein levels were quantified using cellulose-acetate membrane electrophoresis.</p><p>Results: The plasma of animals from the tap water and CCT groups was clear, whereas that of animals from the soybean oil group was cloudy and milky. Moreover, we observed an increase in plasma triglyceride and chylomicron levels in animals from the soybean oil group (though not in animals of the CCT group).</p><p>Conclusions: In this study, we determined the experimental conditions for understanding the absorption and transport of long-chain fatty acid‒rich soybean oil and medium-chain fatty acid‒rich CCT and the functions of lipoproteins, such as chylomicrons, in these processes.</p>

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