Preparation and Characterization of Humic Substances in Peat

DOI
  • Tachibana Yoko
    Laboratory of Medicinal and Biochemical Analysis, Faculty of Pharmaceutical Science, Hiroshima International University
  • Tawa Riichi
    Laboratory of Medicinal and Biochemical Analysis, Faculty of Pharmaceutical Science, Hiroshima International University

Bibliographic Information

Other Title
  • ピートモス中の腐植物質調製と物理化学的性質

Abstract

<p>Humic substances (HS) are natural organic matters originated from plants which have been formed during the microbial degradation in soil and water. They show physical, chemical and biological activities and have been used for the soil supplement and the means for the purification of environment. The effects for the growth of plants and the thermal effects of peat have been also reported. In this study, HS were prepared from peat and were characterized by total polyphenol contents, UV-VIS and IR spectral analysis and high performance size exclusion chromatography (HPSEC), to improve the usefulness in the field of medicine. According to the IHSS (International Humic Substances Society) method, humic acid (HA) fraction and fulvic acid (FA-1~4) fractions were prepared. The yields of HA and FAs were 7.9 % and 0.2 %, respectively. The total polyphenol contents of HA (400 mg/g) was higher than those of FA-1~3 (260, 300, and 350 mg/g, respectively). The UV-VIS spectrum of HA showed λmax at 215 nm and shoulder at 280 nm, on the other hand, those of FA-1~3 showed λmax at 210~215 nm and shoulder at 330 nm. Based on the analysis of HPSEC, the molecular weight of HA distributed wide range (102~104) than FAs (103). Since both of HA and FAs contain polyphenol, they are expected as antioxidants.</p>

Journal

Details 詳細情報について

  • CRID
    1390011318888924544
  • DOI
    10.51029/jtnrs.22.0_111
  • ISSN
    24366617
    13462334
  • Text Lang
    ja
  • Data Source
    • JaLC
  • Abstract License Flag
    Allowed

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