Report on the immunostaining survey at the 2021 Chubu Area Pathology and Cytology Seminar: Immunohistochemical staining of p53 antibody in the Chubu area

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  • SATO Hiaki
    Department of Medical Technology and Clinical Engineering, Faculty of Health and Medical Sciences, Hokuriku University
  • TAMANO Yuko
    Department of Diagnostic Pathology, Kanazawa University Hospital
  • SASAKI Norio
    Department of Clinical Labolatory, Kanazawa Red Cross Hospital
  • YOSHINO Atsuko
    Department of Clinical Labolatory, Public Matto Ishikawa Central Hospital
  • SAKO Kinji
    Department of Clinical Laboratory, JA Aichi Koseiren Chita Kosei Hospital
  • SAKANE Junichi
    Department of Clinical Laboratory, Shizuoka Children’s Hospital
  • ASANO Atsushi
    Ogaki Municipal Hospital

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Other Title
  • 2021年度中部圏支部病理細胞検査研修会における免疫染色サーベイの実施報告―中部圏支部6県におけるp53抗体の免疫染色の実施状況について―
  • 2021ネンド チュウブケン シブ ビョウリ サイボウ ケンサ ケンシュウカイ ニ オケル メンエキ センショク サーベイ ノ ジッシ ホウコク : チュウブケン シブ 6ケン ニ オケル p53 コウタイ ノ メンエキ センショク ノ ジッシ ジョウキョウ ニ ツイテ

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Abstract

<p>Immunostaining aids in pathological diagnosis and selection of therapeutic agents. The Chubu Branch of Japanese Association of Medical Technologists conducts an annual immunostaining survey with the aim improving techniques of histopathological analysis. Unstained specimens labeled with cultured cell lines expressing the p53 protein were distributed to 71 participating institutions, and the specimens and process questionnaires were collected after immunostaining was performed. Members of a committee for evaluating Ishikawa cell immunostaining visually assessed the specimens: staining scores of 4.0–3.7 points were optimal, those of 3.6–3.4 points were good, those of 3.3–3.1 points were acceptable, and those of points below 3.0 indicated that more effort was required. Optimal or good scores were judged as not interfering with diagnosis. Of the 71 participating centers, 59 (83%) had optimal or good staining techniques and no diagnostic problems, whereas four centers required immediate improvement. Only 41 laboratories (62%) followed the recommended staining process, which suggests that the results may be affected by the combination of manufacturers of primary antibodies with various types of automated staining equipment and detection systems. Clones of primary antibodies, management methods, models of automated staining equipment, and differences in detection systems at each institution affect immunostaining results, and it is difficult to eliminate interinstitutional differences even if techniques and processes are standardized. However, we believe that quality assurance and standardization of immunostaining will be realized if these surveys are conducted regularly, the results of facilities are reviewed, the current situation is understood, and the results are examined and improved.</p>

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