Role of calcium signals in circulatory failure and spinal curvature caused by perfluorinated alkyl substances in developing zebrafish
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- LEE Jae Seung
- Laboratory of Toxicology, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
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- MATSUMOTO Kentaro
- Laboratory of Toxicology, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
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- KAWAI Yusuke
- Laboratory of Toxicology, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
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- ISHIBASHI Hiroshi
- Graduate School of Agriculture, Ehime University
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- KUBOTA Akira
- Laboratory of Toxicology, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
Bibliographic Information
- Other Title
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- 発達期ゼブラフィッシュにおける有機フッ素化合物誘発性循環障害および脊柱彎曲に対するカルシウムシグナルの役割
Abstract
<p>Perfluorinated alkyl substances (PFASs) have been widely used for consumer products such as water and grease repellents, household products, electronics, and food contact materials. Some of PFASs have been and are supposed to be listed as persistent organic pollutants (POPs) due to their persistent and bioaccumulative properties, as well as toxicity. The present study aimed at evaluating developmental toxicity of representative PFASs and understanding the possible mechanisms using zebrafish embryos. Embryos continuously exposed to perfluorooctane sulfonate (PFOS) and perfluorohexane sulfonate (PFHxS) elicited pericardial edema and reduction of blood flow in trunk vessels at 96 hours post fertilization in a concentration-dependent manner, with greater potency observed in PFOS than in PFHxS. PFOS also elicited spinal curvature. RNA sequencing revealed that genes involved in calcium-related signaling (e.g., pth1a, fbxo32, nos2a, tcap, and tnni4b.1) and in metabolic process (e.g., nos2a, mlycd, ppp1r3aa, pdk2b, lepa, and lepb) were significantly and commonly affected by PFOS (10 μM) and PFHxS (100 μM). Co-exposure to PFOS and NMDA receptor antagonists (memantine and MK-801) rescued circulatory failure and spinal curvature caused by PFOS. In contrast, PFOS-induced circulatory failure and spinal curvature were exacerbated by co-exposure to a PPARα antagonist (MK-886). These results suggest that circulatory failure and spinal curvature caused by PFOS and possibly by PFHxS might be through disruption of calcium signaling, and PPARα signaling may have a protective role against it.</p>
Journal
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- Annual Meeting of the Japanese Society of Toxicology
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Annual Meeting of the Japanese Society of Toxicology 49.1 (0), P-74S-, 2022
The Japanese Society of Toxicology
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Details 詳細情報について
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- CRID
- 1390011716213288064
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- Text Lang
- ja
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- Data Source
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- JaLC
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- Abstract License Flag
- Disallowed