Morphological Changes in Mitochondria, Mitochondrial Nucleoids, and Actin Cytoskeleton during the Transition from Anaerobic to Aerobic Culture in the Yeast <i>Saccharomyces cerevisiae</i>

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  • Kito Yumiko
    Department of Biology, Faculty of Science, Yamaguchi University
  • Taku Mikari
    Department of Biology, Faculty of Science, Yamaguchi University
  • Miyakawa Isamu
    Department of Biology, Faculty of Science, Yamaguchi University

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<p>We examined the morphological changes in mitochondria, mitochondrial nucleoids (mt-nucleoids), and the actin cytoskeleton in the yeast Saccharomyces cerevisiae during the transition from anaerobic to aerobic culture by immunofluorescence microscopy, DAPI staining, and rhodamine-phalloidin staining. The cells grown anaerobically to the stationary phase contained a small number of mitochondria, 7.3 per cell on average. When cells were transferred to aerobic culture, mitochondria began to elongate within 1 h and divided into smaller spherical mitochondria within 8 h. The number of mitochondria per cell on average was 29.4 at 4 h, 52.8 at 6 h, and 71.8 at 8 h. These morphological changes in mitochondria required respiratory activity and coincided well with those of the mt-nucleoids. In addition, we found that the rapid conversion of the cortical actin patches from the large aggregates to the small patches occurred during the transition from anaerobic to aerobic culture.</p>

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