Compound-specific Nitrogen Isotope and Radiocarbon Analyses of Amino Acids in Biological Samples

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  • ISHIKAWA Naoto F.
    Biogeochemistry Research Center, Japan Agency for Marine-Earth Science and Technology

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Other Title
  • 生物試料中のアミノ酸の窒素安定同位体比及び放射性炭素濃度測定

Abstract

<p>This article is a summary of three of the author’s previous studies12)20)29). Compound-specific isotope analysis of nitrogen (δ15N) and radiocarbon (Δ14C) analyses of amino acids (AAs) have significantly contributed to environmental sciences such as anthropology, biogeochemistry, and ecology. To study mixed ecosystems where basal resources are derived from both terrestrial and aquatic primary producers, the δ15N of methionine (δ15NMet) was determined. The results showed that the difference in δ15N between methionine and phenylalanine in terrestrial ecosystems (ΔMet–Phe = –16.5±0.5‰) is clearly distinguishable from that in aquatic ecosystems (ΔMet–Phe = –5.0±0.5‰). Next, AA δ15N values were systematically compared among three methods using gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS), preparative liquid chromatography (LC) separation followed by elemental analyzer/IRMS (LC × EA/IRMS), and LC separation followed by GC/C/IRMS (LC × GC/C/IRMS). The δ15N values of glutamic acid (δ15NGlu) and Phe (δ15NPhe) in fish muscle were fairly consistent between all pairs of methods. Finally, the isolation and purification method of AAs for Δ14C analysis was improved. The AA Δ14C values determined by EA/accelerator mass spectrometry (EA/AMS) were in strong agreement with the consensus Δ14C value of each amino acid standard.</p>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 71 (12), 653-662, 2022-12-05

    The Japan Society for Analytical Chemistry

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