Analysis of Free Pantothenic Acid in Foods by HPLC and LC-MS/MS
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- Horie Masakazu
- Department of Food Science, Faculty of Home Economics, Otsuma Women’s University
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- Watanabe Moe
- Department of Food Science, Faculty of Home Economics, Otsuma Women’s University
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- Tada Atsuko
- Division of Food Additives, National Institute of Health Sciences
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- Sato Kyoko
- Division of Food Additives, National Institute of Health Sciences
Bibliographic Information
- Other Title
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- HPLCおよびLC-MS/MSによる食品中の遊離型パントテン酸の分析
- HPLC オヨビ LC-MS/MS ニ ヨル ショクヒン チュウ ノ ユウリガタ パントテンサン ノ ブンセキ
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Abstract
<p>A simple and reliable analytical method has been developed for the determination of pantothenic acid in food. For the high-protein food, 20 mL of water was added to 2 g of sample, and after homogenization extraction, 1 mL of 15% zinc sulfate solution was added, mixed well, centrifuged, and the supernatant was filtered to make the test solution. For the low-protein food, 20 mL of 1% formic acid solution was added to 2 g of sample, homogenized, extracted, centrifuged, and the supernatant was filtered to make the test solution. The HPLC separation was carried out on a L-column2 ODS column with 0.02 mol/L phosphate solution (pH 3.0)- acetonitrile (95 : 5) as the mobile phase, and detected at 200 nm. The LC-MS/MS conditions were L-column2 ODS as the separation column, 5 mmol/L ammonium formate (containing 0.01% formic acid)-methanol (85 : 15) as the mobile phase, and multiple reaction monitoring (MRM) was used for detection. The recoveries of pantothenic acid in milk powder and nutritional food products were more than 88% with high precision. As a result of analyzing commetrcially available foods labeled as containing pantothenic acid, analytical values almost identical to the labeled values were obtained, and a high correlation was observed between the values obtained by HPLC and LC-MS/MS.</p>
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 64 (1), 47-52, 2023-02-25
Japanese Society for Food Hygiene and Safety
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Details 詳細情報について
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- CRID
- 1390013795251964928
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- NII Book ID
- AN00117741
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 032729869
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
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- Abstract License Flag
- Disallowed