Real-time PCR assay for detection of Environmental DNA from <i>Chironex yamaguchii</i>
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- Azama Yasuhito
- Okinawa Prefectural Institute of Health and Environment
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- Oka Shin-ichiro
- Okinawa Churashima Foundation
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- Teruya Morimi
- Okinawa Prefectural Institute of Health and Environment
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- Toshino Sho
- Kuroshio Biological Research Foundation
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- Tanimoto Miyako
- Okinawa Churashima Foundation
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- Hanahara Nozomi
- Okinawa Churashima Foundation
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- Kuba Yumani
- Okinawa Prefectural Institute of Health and Environment
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- Miyagi Ayano
- Okinawa Prefectural Institute of Health and Environment
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- Fukuchi Yoshimune
- Miyako Regional Public Health Center
Bibliographic Information
- Other Title
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- ハブクラゲの環境DNAを検出するためのリアルタイムPCRアッセイ
Abstract
<p>The box jellyfish Chironex yamaguchii is a highly venomous cubomedusa, also known as “habu-kurage” in Japan. It is a significant threat to marine activities in Okinawa in southern Japan. Annually, envenomation of the species causes nearly 100 to 200 injuries in the Okinawa Prefecture. The present study developed a species-specific real-time PCR assay to analyze environmental DNA (eDNA) for rapid detection of jellyfish in water samples. Primers and probe were designed based on the sequences of the mitochondrial cytochrome c oxidase subunit I region using tissue samples of nine specimens obtained from the Okinawa Prefecture. The specificity of the real-time PCR assay was confirmed by amplifying the DNA samples of 10 different box jellyfish species. A practical assay for eDNA detection was successful on water samples obtained from the natural habitat of C. yamaguchii. In addition, the degradation rate of eDNA in the tank experiment was 65% of the initial concentration at 1 h, 22% at 12 h, and 3% at 48 h.</p>
Journal
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- Medical Entomology and Zoology
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Medical Entomology and Zoology 74 (1), 13-20, 2023-03-25
The Japan Society of Medical Entomology and Zoology
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Keywords
Details 詳細情報について
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- CRID
- 1390014093884817408
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- ISSN
- 21855609
- 04247086
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- KAKEN
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- Abstract License Flag
- Disallowed