Verification of improvement of periorbital wrinkles by using Asakado Skin Care

The present study conducted a trial for a skin application of a test cosmetic product with 34 healthy women aged between 35 to 55 years of age (43.4 ± 0.6) who satisfied the classification of 1, 2, and 3 in the standards for the wrinkle grade assessment in accordance with "Guidelines for Evaluation of Cosmetic Functions". In a half face method for 8 weeks, the test cosmetic sample was applied to one lateral periorbital lines of a face (applied side) twice a day and no treatment was applied over the other periorbital lines (non-applied side). The test product was a skin-care cosmetic that contained diverse constituents such as Ceratonia siliqua L. fruit extract, Polygonum tinctorium leaf/stem extract, and ribosomal constituents. In test results after 8 weeks for the primary endpoint of grade assessments of periorbital wrinkles, the grade on the applied side indicated significant improvements through a visual assessment during a medical examination and a visual assessment of observing case photographic images by medical specialists. However, results of the primary endpoint on the non-applied side also indicated significant improvements. There was no significant difference between two sides. Replica analysis examining periorbital wrinkles did not suggest any significant changes between on applied or non-applied side as well as between before and after test product application. Secondary endpoint of skin viscoelasticity (R2) significantly improved on the applied and non-applied sides after 8 weeks. There was no significant difference of changes in quantity. In the process of the trial, no adverse event was reported and the safety of the test product was confirmed. We conducted a further investigation to explore possibilities that diverse functional ingredients contained in this product were absorbed through percutaneous absorption with an assistance of ribosomal constituents, and exerted effects toward the other side of the face. To verify this hypothesis, further examinations are required to confirm blood concentrations of active components after tests.