Oxidative stress and cellular toxicity induced by dihydropyrazine: a comparative study with other Maillard reaction products
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- Miyauchi Yuu
- Laboratory of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Sojo University
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- Koba Haruna
- Laboratory of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Sojo University
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- Sawai Madoka
- International University of Health and Welfare, School of Pharmacy at Fukuoka
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- Kansui Hisao
- Laboratory of Organic Chemistry, Faculty of Pharmaceutical Sciences, Sojo University
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- Takechi Shinji
- Laboratory of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Sojo University
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<p>Glycation products are generated during the Maillard reaction, a non-enzymatic reaction between reducing sugars and the amino groups of proteins, which accumulate in the body with aging and cause many diseases. Herein, we have focused on dihydropyrazines (DHPs), which are glycation products formed by the dimerization of D-glucosamine or 5-aminolevulinic acid, and have reported that DHPs can produce several kinds of radicals and induce cytotoxicity via oxidative stress. To advance our understanding of DHP-mediated cytotoxicity, we selected a DHP, 3-hydro-2,2,5,6-tetramethylpyrazine (DHP-3), and two major Maillard reaction products, Nε-(carboxymethyl)-L-lysine (CML) and acrylamide, and performed comparative experiments focusing on their cytotoxicity and their ability to induce oxidative stress. The order of increasing cytotoxicity was DHP-3, acrylamide, and CML, and the LC50 value could be calculated only for DHP-3 (0.53 mM), indicating that DHP-3 is more toxic than the other Maillard reaction products. However, their toxicities were significantly lower than those of common toxic chemicals. Further, the results of their cytotoxicity assay were consistent with the results of intracellular reactive oxygen species production and activation of oxidative stress response signaling. These results indicate that the acute toxicity of Maillard reaction products is closely related to their ability to induce oxidative stress, and that DHP-3 is a particularly strong inducer of oxidative stress and thus exhibits high cytotoxicity among Maillard reaction products. In addition, we have shown that a comprehensive analysis comparing multiple Maillard reaction products is effective for elucidating their complex and diverse toxicities.</p>
収録刊行物
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 48 (5), 263-272, 2023
一般社団法人 日本毒性学会
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詳細情報 詳細情報について
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- CRID
- 1390014481303451136
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- NII書誌ID
- AN00002808
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- ISSN
- 18803989
- 03881350
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- NDL書誌ID
- 032944446
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- PubMed
- 37121741
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
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- 使用不可