Evaluation of cold atmospheric pressure plasma irradiation of water as a method of singlet oxygen generation

DOI Web Site 参考文献42件
  • Takajo Tokuko
    Faculty of Pharmaceutical Sciences, Nihon Pharmaceutical University
  • Nagahama Hiroki
    Faculty of Pharmaceutical Sciences, Nihon Pharmaceutical University
  • Zuinen Katsuya
    Faculty of Pharmaceutical Sciences, Nihon Pharmaceutical University
  • Tsuchida Kazunori
    Faculty of Pharmaceutical Sciences, Nihon Pharmaceutical University
  • Okino Akitoshi
    Laboratory for Future Interdisciplinary Research of Science and Technology, Institute of Innovative Research, Tokyo Institute of Technology
  • Anzai Kazunori
    Faculty of Pharmaceutical Sciences, Nihon Pharmaceutical University

抄録

<p>We used cold atmospheric pressure plasma jet to examine in detail 1O2 generation in water. ESR with 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide, a secondary amine probe, was used for the detection of 1O2. Nitroxide radical formation was detected after cold atmospheric pressure plasma jet irradiation of a 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide solution. An 1O2 scavenger/quencher inhibited the ESR signal intensity induced by cold atmospheric pressure plasma jet irradiation, but this inhibition was not 100%. As 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide reacts with oxidizing species other than 1O2, it was assumed that the signal intensity inhibited by NaN3 corresponds to only the nitroxide radical generated by 1O2. The concentration of 1O2 produced by cold atmospheric pressure plasma jet irradiation for 60 ‍s was estimated at 8 ‍μM. When this 1O2 generation was compared to methods of 1O2 generation like rose bengal photoirradiation and 4-methyl-1,4-etheno-2,3-benzodioxin-1(4H)-propanoic acid (endoperoxide) thermal decomposition, 1O2 generation was found to be, in decreasing order, rose bengal photoirradiation ≥ cold atmospheric pressure plasma jet > endoperoxide thermal decomposition. Cold atmospheric pressure plasma jet is presumed to not specifically generate 1O2, but can be used to mimic states of oxidative stress involving multiple ROS.</p>

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