Effects of Cathepsin A on the Cytodifferentiation of Periodontal Ligament Cells
-
- Jirouta KITAGAKI
- Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry Aijinkai Rehabilitation Hospital
-
- Masahiro MATSUMOTO
- Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
-
- Chiharu FUJIHARA
- Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
-
- Hiromi SAKASHITA
- Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
-
- Asae HIRAI
- Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
-
- Motozo YAMASHITA
- Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
-
- Masahiro KITAMURA
- Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
-
- Shinya MURAKAMI
- Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
Bibliographic Information
- Other Title
-
- 歯根膜細胞の骨芽細胞分化におけるカテプシンAの影響
Search this article
Abstract
<p> Purpose: The purpose of this study was to understand the effects of cathepsin A on the cytodifferentiation of periodontal ligament cells.</p><p> Methods: Expression of cathepsin A was assessed by RT-PCR and immunoblotting in mouse periodontal ligament cells (MPDL22). MPDL22 were incubated with cathepsin A inhibitor ebelactone B and calcification-related gene expression was assessed by real-time PCR. To evaluate the genetic risk factors of cathepsin A for aggressive periodontitis in a Japanese population, we utilized an exome sequencing database.</p><p> Results: Cathepsin A was expressed in MPDL22. Ebelactone B clearly inhibited the expression of calcification-related genes osteopontin, osteonectin, and type Ⅰ collagen in MPDL22. Two single nucleotide polymorphisms (SNP) in cathepsin A, rs181943893 (c.54G>C, p.Leu18=) and rs72555383 (c.33GCT [7], p.Leu19del), showed significantly different minor allele frequency through case-control exome sequencing.</p><p> Conclusions: We found that the cathepsin A inhibitor ebelactone B inhibited the cytodifferentiation of periodontal ligament cells. We also found that SNP rs181943893 and rs72555383 were genetic risk factors for aggressive periodontitis in a Japanese population, indicating that cathepsin A plays an important role in maintaining the homeostasis of periodontal ligament tissues.</p>
Journal
-
- The Japanese Journal of Conservative Dentistry
-
The Japanese Journal of Conservative Dentistry 66 (4), 214-223, 2023-08-31
The Japanese Society of Conservative Dentistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390015819046679808
-
- ISSN
- 21880808
- 03872343
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
-
- Abstract License Flag
- Disallowed