Effects of Cathepsin A on the Cytodifferentiation of Periodontal Ligament Cells

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  • Jirouta KITAGAKI
    Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry Aijinkai Rehabilitation Hospital
  • Masahiro MATSUMOTO
    Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
  • Chiharu FUJIHARA
    Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
  • Hiromi SAKASHITA
    Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
  • Asae HIRAI
    Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
  • Motozo YAMASHITA
    Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
  • Masahiro KITAMURA
    Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry
  • Shinya MURAKAMI
    Department of Periodontology and Regenerative Dentistry, Osaka University Graduate School of Dentistry

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Other Title
  • 歯根膜細胞の骨芽細胞分化におけるカテプシンAの影響

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Abstract

<p> Purpose: The purpose of this study was to understand the effects of cathepsin A on the cytodifferentiation of periodontal ligament cells.</p><p> Methods: Expression of cathepsin A was assessed by RT-PCR and immunoblotting in mouse periodontal ligament cells (MPDL22). MPDL22 were incubated with cathepsin A inhibitor ebelactone B and calcification-related gene expression was assessed by real-time PCR. To evaluate the genetic risk factors of cathepsin A for aggressive periodontitis in a Japanese population, we utilized an exome sequencing database.</p><p> Results: Cathepsin A was expressed in MPDL22. Ebelactone B clearly inhibited the expression of calcification-related genes osteopontin, osteonectin, and type Ⅰ collagen in MPDL22. Two single nucleotide polymorphisms (SNP) in cathepsin A, rs181943893 (c.54G＞C, p.Leu18=) and rs72555383 (c.33GCT [7], p.Leu19del), showed significantly different minor allele frequency through case-control exome sequencing.</p><p> Conclusions: We found that the cathepsin A inhibitor ebelactone B inhibited the cytodifferentiation of periodontal ligament cells. We also found that SNP rs181943893 and rs72555383 were genetic risk factors for aggressive periodontitis in a Japanese population, indicating that cathepsin A plays an important role in maintaining the homeostasis of periodontal ligament tissues.</p>

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