Development of Analytical Method and Surveillance ofGibberellic Acid in Banana, Cherry, and Kiwi Fruit

Yamasaki Yuki, Suzuki Yoshinari, Kitayama Ikuko, Nunome Mari, Kondo Midori, Sakai Takatoshi, Nemoto Satoru, Akiyama Hiroshi, Tsutsumi Tomoaki

doi:10.3358/shokueishi.64.123

<p>Gibberellic acid (GA₃) is commonly used as a plant growth regulator in many food crops owing to its essential signaling functions during plant growth and development. In Japan, a threshold for administrative action for GA₃ content of 0.3 mg/kg applies in produce in which maximum residue limits have not been established. Although the threshold is based on previous studies, the GA₃ concentrations in individual foods are still unknown. Thus, we surveyed the concentrations of GA₃ in banana, cherry, and kiwi fruit on the Japanese market. We developed and validated a method for the analysis of GA₃ using solid-phase extraction and LC-MS/MS in accordance with accepted criteria of trueness, repeatability, and selectivity. The limits of detection and of quantification were determined as 0.005 and 0.05 mg/kg, respectively, in all fruits. Concentrations of GA₃ did not exceed 0.3 mg/kg regardless of ripeness, suggesting the reasonability of the current regulation of GA₃ in banana, cherry, and kiwi fruit. These findings can support prompt administrative action on these fruits, contributing to the regulation of GA₃ in Japan.</p>

Development of Analytical Method and Surveillance ofGibberellic Acid in Banana, Cherry, and Kiwi Fruit

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