Population Analysis of Self-reproducing Giant Vesicle Consisting of Cationic Amphiphiles towards Model-protocell Providing Evolvability

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  • 進化するベシクル型人工細胞の基盤となるカチオン性自己生産ベシクルの集団計測
  • シンカ スル ベシクルガタ ジンコウ サイボウ ノ キバン ト ナル カチオンセイ ジコ セイサン ベシクル ノ シュウダン ケイソク

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Using Z-stack observations of laser scanning confocal fluorescence microscopy, population analysis of self-reproducing cationic giant vesicles (GVs) dispersing into buffered saline for an enzymatic reaction, which is the major component of GV-based model-protocell, was performed. After addition of the precursor of the membrane molecule, the number of GVs showd a 4-times increase, and the diameters of GVs increased from 1-2 to 3-4 μm before addition of the precursor . This behavior can be explained by a cell-like auxesis and self-division of the self-reproducing GVs caused by the chemical reaction to produce the membrane molecule constructing GVs themselves from the membrane precursor assisted by the lipophilic catalyst placed into the vesicular membrane. This interpretation is consistent with a previous report employing a combination of optical microscopy observation and flow-cytometry (FCM). This new analysis, which can be performed using only optical microscopy and not FCM, which requires special experimental conditions optimizing GVs, facilitates rapid measurement for optimization of the conditions for construction of the GV-based model protocell, which can evolve spontaneously.

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