Quantitative Determination Method of Aconitum Monoester Alkaloids Using Relative Molar Sensitivity (RMS) for the Assay in the Japanese Pharmacopoeia
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- Uchiyama Nahoko
- National Institute of Health Sciences
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- Hosoe Junko
- National Institute of Health Sciences
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- Ishizuki Kyoko
- National Institute of Health Sciences
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- Arai Ryoko
- National Institute of Health Sciences
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- Sugimoto Naoki
- National Institute of Health Sciences
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- Suzuki Azusa
- Tsumura & Co.
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- Asano Ryuji
- Tsumura & Co.
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- Igarashi Yasushi
- Tsumura & Co.
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- Miura Toru
- FUJIFILM Wako Pure Chemical Corporation
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- Muto Yasuhiro
- FUJIFILM Wako Pure Chemical Corporation
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- Suematsu Takako
- JEOL Ltd.
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- Komatsu Takanori
- JEOL Ltd.
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- Higano Taro
- Taisho Pharmaceutical Co., Ltd.
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- Furukawa Takun
- Taisho Pharmaceutical Co., Ltd.
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- Shimada Norimoto
- Tokiwa Phytochemical Co., Ltd.
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- Goda Yukihiro
- National Institute of Health Sciences
Bibliographic Information
- Other Title
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- ブシモノエステルアルカロイドの相対モル感度（RMS）を用いた日本薬局方定量法の検討
Abstract
<p>Recently, a novel quantitative method using relative molar sensitivity (RMS) was applied to quantify the ingredients of drugs and foods. An important development in this regard can be observed in the Japanese Pharmacopoeia (JP) 18, where the quantification of perillaldehyde, an unstable compound, in crude drug “Perilla Herb,” was revised to incorporate the RMS method. In this study, the primary objective was to improve the tester safety and reduce the amount of reagents used in the JP test. To achieve this, the quantification of three toxic Aconitum monoester alkaloids (AMAs) was explored using the RMS method, employing a single reference compound for all three targets. These AMAs, namely benzoylmesaconine hydrochloride, benzoylhypaconine hydrochloride, and 14-anisoylaconine hydrochloride, which are the quantitative compounds of Kampo extracts containing Aconite Root (AR), were quantified using the reference compound benzoic acid (BA). Reliable RMS values were obtained using both 1H-quantitative NMR and HPLC/UV. Using the RMS of three AMAs relative to the BA, the AMA content (%) in commercial AMAs quantitative reagents were determined without analytical standards. Moreover, the quantitative values of AMAs using the RMS method and the calibration curve method using the three analytical standards were similar. Additionally, similar values were achieved for the three AMAs in the Kampo extracts containing AR using the RMS and the modified JP18 calibration curve methods. These results suggest that the RMS method is suitable for quantitative assays of the Kampo extracts containing AR and can serve as an alternative to the current method specified in the JP18.</p>
Journal
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- YAKUGAKU ZASSHI
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YAKUGAKU ZASSHI 143 (11), 951-962, 2023-11-01
The Pharmaceutical Society of Japan
