A STUDY ON THE DISTRIBUTION CHARACTERISTICS OF ENVIRONMENTAL DNA ASSOCIATED WITH SEAGRSS BEDS AND THE SAMPLING AREA SELECTION PROCEDURE

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  • 藻場を対象とした環境DNAの分布傾向と採水範囲の検討方法について

Abstract

<p> In water areas, environmental DNA (eDNA) monitoring is expected to be a useful method to collect biological information at single/multiple locations and continuously over the long-term, because it only requires sampling a small amount of water. However, in marine areas, it has been reported that the amount of eDNA varies depending on the sampling area and the sampling timing during a day with the tidal current variation. Therefore, in eDNA sampling, it is important to understand the influence of tidal currents. In this study, sea water samplings were conducted in order to understand the characteristics of the spatial and temporal distribution of eDNA associated with eelgrass beds, which are adherent organisms. The distribution of eDNA along the cross-shore direction were observed and the temporal change in the amount of eDNA were confirmed. The results showed that the distribution range of eDNA was approximately 100m wider than that of the eelgrass beds and the distribution varied with the currents. Additionally, the simulation with advection-diffusion analysis were conducted, where the amount of eDNA was represented with concentration value of virtual substance to qualitatively reproduce the spatial and temporal distribution. The simulations were conducted with the different current conditions, and it is suggested that spatially/temporally-appropriate sampling points can be selected.</p>

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