Evaluation of the impact of carryover in a fully automated, high-sensitivity hepatitis B core-related antigen assay

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  • Ohne Kumiko
    Clinical Technology Department, Clinical Laboratory, Nagoya City University Hospital
  • Kani Satomi
    Clinical Technology Department, Clinical Laboratory, Nagoya City University Hospital
  • Kitamura Yoshiyuki
    Research and Development Division, Fujirebio Inc.
  • Sagi Haruka
    Research and Development Division, Fujirebio Inc.
  • Kaneko Atsushi
    Research and Development Division, Fujirebio Inc.
  • Ohhashi Minoru
    Clinical Technology Department, Clinical Laboratory, Nagoya City University Hospital
  • Kikuchi Shohei
    Department of Clinical Laboratory Medicine, Nagoya City University Hospital Department of Cardiology, Nagoya City University Graduate School of Medical Sciences
  • Tanaka Yasuhito
    Department of Gastroenterology and Hepatology, Faculty of Life Sciences, Kumamoto University
  • Inoue Takako
    Department of Clinical Laboratory Medicine, Nagoya City University Hospital

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Other Title
  • 高感度HBコア関連抗原全自動測定系におけるキャリーオーバーの検証
  • コウカンド HB コア カンレン コウゲン ゼン ジドウ ソクテイケイ ニ オケル キャリーオーバー ノ ケンショウ

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Abstract

<p>We developed and reported a fully automated, high-sensitivity hepatitis B core-related antigen assay (iTACT-HBcrAg). This study aimed to evaluate the impact of carryover during the use of iTACT-HBcrAg by analyzing samples with high HBcrAg levels. A negative sample tested immediately after a sample with an HBcrAg of 8.7 LogU/mL showed an HBcrAg of 2.4 LogU/mL, thus confirming carryover. The sample with an HBcrAg of 8.8 LogU/mL and collection tubes containing the negative samples were continuously opened to check for the effects of sample splashing and aerosols. The results showed that sample splashing and aerosols did not account for the carryover. The iTACT-HBcrAg is a highly sensitive assay; therefore, carryover can occur when magnetic particle-antigen complexes are introduced into the next sample via the BF wash nozzle. Any sample determined to be positive for HBcrAg after the testing of a sample with an HBcrAg above the measurement range should be retested for confirmation.</p>

Journal

  • Kanzo

    Kanzo 64 (12), 656-659, 2023-12-01

    The Japan Society of Hepatology

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