Analysis of oxidative stress sensitivity of various immune cells using redox state monitoring mice

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  • 酸化還元状態モニターマウスを用いた各種免疫担当細胞の酸化ストレス感受性解析

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<p>Background: Oxidative stress is involved in many age-associated diseases, as well as in the aging process. Although reactive oxygen species (ROS) were thought to be primarily cytotoxic, recent studies indicate that ROS are important for cell function. In the immune cells, ROS signal appear to be essential for the activation of immune cells. We have generated transgenic mice expressing redox-sensitive green fluorescent proteins (roGFPs) (Biochim Biophys Acta Gen Subj. 1867, 130302, 2023). Two types of roGFP mice were constructed: CRO and MRO mice, which measure the redox state of whole cells and mitochondria, respectively. In this study, we measured the redox state of various immune cells using roGFP mice and analyzed their susceptibility to oxidative stress.</p><p>Methods: Immune cells isolated from CRO and MRO mice were stained with cell surface markers for B cells, T cells, NK cells, neutrophils, and eosinophils. The redox state of immune cells was measured by flow cytometry. In addition, the cells were treated with various concentrations of hydrogen peroxide, and the susceptibility to oxidative stress of various immune cells was analyzed.</p><p>Results: The basal redox state of each cell type differed significantly. The susceptibility to oxidative stress was also differed dramatically among cell types. Furthermore, redox state differed considerably between CRO and MRO mice. Monitoring redox state using a mouse model of the disease should be a powerful and convenient tool for the elucidation of the activation mechanism of immune cells from a new perspective.</p>

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