書誌事項
- タイトル別名
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- Dispensable role of Rac1 and Rac3 after cochlear hair cell specification
抄録
<p>The Rho-family GTPases play various functions, including cytoskeletal regulation and cell polarity establishment. Using Cdc42-KO mice under the control of the Atoh1 promoter (Atoh1-Cre;Cdc42flox/flox), we previously reported that Cdc42 plays essential roles in the maintenance of cochlear hair cells (HCs) after HC specification. Although Rac1 and Rac3 have been reported to play important roles during embryonic development of the inner ear, little is known regarding their function in cochlear HCs after specification. Here, we revealed the localization and activation of Racs at stereocilia and apical junctional complexes in primary cochlear HCs using gene gun-mediated transfection of GFP-tagged Rac plasmids and transgenic mice expressing a Rac1- fluorescence resonance energy transfer (FRET) biosensor. To examine the roles of Racs after cochlear HC specification at individual levels, we generated conditional Rac-KO mice: Atoh1-Cre;Rac1flox/flox (Rac1-KO) and Atoh1-Cre;Rac1flox/flox;Rac3−/− (Rac1/Rac3-DKO) mice. Unexpectedly, both Rac1-KO and Rac1/Rac3-DKO mice exhibited normal cochlear HC morphology and normal hearing function. No hearing vulnerability was observed in young adult Rac1/Rac3-DKO mice even after intense noise exposure. Taken together, although Rac1 and Rac3 contribute to the early development of sensory epithelia in cochleae, they are dispensable after cochlear HC specification.</p>
収録刊行物
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- 日本薬理学会年会要旨集
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日本薬理学会年会要旨集 97 (0), 2-B-O07-2-, 2023
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390017267762189056
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- ISSN
- 24354953
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
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- 抄録ライセンスフラグ
- 使用不可