書誌事項
- タイトル別名
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- Involvement of P2X4 receptor in osteoblast differentiation
説明
<p>Osteoblast growth and differentiation, which are controlled by Ca2+ signaling, are important for bone formation and homeostasis. ATP released from hemichannels, such as pannexins and connexins located within the cell membrane, also contributes to osteoblast differentiation. The purpose of this study is to elucidate the functional relationship between hemichannel-mediated ATP efflux and P2X receptor in osteoblast differentiation of the osteoblastic cell line MC3T3-E1, established from mouse calvaria. </p><p>We found that the pharmacological blockade of hemichannels decreased the amount of released ATP from MC3T3-E1 cells during osteoblast differentiation. The expression levels of the alkaline phosphatase ALP, an osteoblast differentiation marker, were also suppressed by the treatment with CBX, a hemichannel blocker and 5-BDBD, a P2X4 blocker. ATP stimulation induced a transient Ca2+ influx through ATP-sensitive Ca2+ permeable channels, and this ATP-stimulated [Ca2+]i rises were attenuated by the treatment with 5-BDBD. In addition, the endochondral ossification of metatarsal bones was inhibited by the treatment with CBX and 5-BDBD. These results suggest that ATP efflux via hemichannels may be involved in P2X4 mediated-ATP signaling during osteoblast differentiation. The functional coupling between hemichannels and P2X4 receptors plays an essential role in maintaining the bone homeostasis via modulating osteoblast differentiation.</p>
収録刊行物
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- 日本薬理学会年会要旨集
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日本薬理学会年会要旨集 97 (0), 3-B-P-002-, 2023
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390017267762303104
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- ISSN
- 24354953
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
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- 抄録ライセンスフラグ
- 使用不可