Deletion of RAMP1 signaling enhances diet-induced obesity and fat absorption via intestinal lacteals in mice
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- Hosono Kanako
- Dept. Pharm., Sch. Med., Kitasato Univ. Dept. Mol. Pharm., Grad. Sch. Med., Kitasato Univ.
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- Ito Yoshiya
- Dept. Pharm., Sch. Med., Kitasato Univ. Dept. Mol. Pharm., Grad. Sch. Med., Kitasato Univ.
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- Betto Tomohiro
- Dept.Gastroenterol, Sch. Med., Kitasato Univ.
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- Tanabe Mina
- Dept. Mol. Pharm., Grad. Sch. Med., Kitasato Univ.
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- Yamashita Atsushi
- Dept. Mol. Pharm., Grad. Sch. Med., Kitasato Univ.
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- Kamata Mariko
- Dept. Pharm., Sch. Med., Kitasato Univ. Dept. Mol. Pharm., Grad. Sch. Med., Kitasato Univ.
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- Hatanaka Koh
- Dept. Pharm., Sch. Med., Kitasato Univ. Dept. Mol. Pharm., Grad. Sch. Med., Kitasato Univ.
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- Majima Masataka
- Dept. Pharm., Sch. Med., Kitasato Univ. Faculty of Health and Medical Sciences, Kanagawa Institute of Technology Fac. Health & Med. Sci., Kanagawa Inst. Tech.
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- Amano Hideki
- Dept. Pharm., Sch. Med., Kitasato Univ. Dept. Mol. Pharm., Grad. Sch. Med., Kitasato Univ.
Bibliographic Information
- Other Title
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- RAMP1シグナルの欠損は、食餌誘発性肥満と腸乳管を介した脂肪吸収を促進する
Abstract
<p>Lacteals play a critical role in the absorption and transport of dietary lipids into the circulation. We examined the role of signaling for receptor activity-modifying protein 1 (RAMP1), a subunit of CGRP receptor, in lacteal morphology and function in response to a high-fat diet (HFD). RAMP1 deficient (RAMP1–/–) or wild-type (WT) mice were fed a normal diet or HFD for 8 weeks. RAMP1–/– mice fed an HFD showed heavier body weights than WT mice fed an HFD, which was associated with high levels of total cholesterol, triglycerides, and glucose. HFD-fed RAMP1–/– mice had shorter length of lacteals and greater width of lacteals than HFD-fed WT mice. HFD-fed RAMP1–/– mice had lower gene expression levels of lymphatic endothelial cell markers including VEGFR3, and lymphatic vascular growth factor VEGF-C than HFD-fed WT mice. The concentration of the absorbed lipid tracer in HFD-fed RAMP1–/– mice was higher than that in HFD-fed WT mice. The zipper-like continuous junctions were predominant in HFD-fed WT mice, while the button-like discontinuous junctions were predominant in HFD-fed RAMP1–/– mice. These results suggest that deletion of RAMP1 signaling suppressed lacteal growth and VEGF-C/VEGFR3 expression and accelerated the uptake and transport of dietary fats through discontinuous junctions of lacteals, leading to excessive obesity.</p>
Journal
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- Proceedings for Annual Meeting of The Japanese Pharmacological Society
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Proceedings for Annual Meeting of The Japanese Pharmacological Society 97 (0), 3-B-P-039-, 2023
Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390017267762376704
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- ISSN
- 24354953
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
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- Abstract License Flag
- Disallowed