イヌ、マウスおよびヒト TRPA1 におけるメントールや低温刺激に対する反応性の違い

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  • Different sensitivity of canine, mouse, and human TRPA1 channels to menthol or cold stimulation

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<p>Transient receptor potential ankyrin 1 (TRPA1) is a Ca2+-permeable, nonselective cation channel that is activated by a wide variety of stimuli. Mouse and human TRPA1 exhibit different reactivity to some stimuli, including chemicals such as menthol as well as cold stimuli. In this study, we analyzed the reactivity of heterologously expressed canine, mouse, and human TRPA1 to menthol or cold stimulation in Ca2+-imaging experiments. Canine and human TRPA1 exhibited a similar response to menthol, namely, activation in a concentration-dependent manner, even at the high concentration range, in contrast to the mouse TRPA1. In addition, the response during the removal of menthol was different; mouse TRPA1 exhibited a typical response with a rapid increase in [Ca2+]i (“off-response”), whereas canine and human TRPA1 responded differently to each other. Finally, canine TRPA1 as well as mouse and human TRPA1 were activated by cold stimulation, although cold sensitivity varied among these species. These responses were suppressed by the selective TRPA1 inhibitor HC-030031. Because the concentration-dependency and “off-response” of menthol as well as the cold sensitivity were not uniform among these species, studies of canine TRPA1 might be useful for understanding the species-specific functional properties of mammalian TRPA1.</p>

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