Identification of the Csr global regulatory system mediated by small RNA decay in <i>Aeromonas salmonicida</i>
-
- Gladyshchuk Olga
- Graduate School of Science and Technology, Niigata University
-
- Yoshida Masaki
- Graduate School of Science and Technology, Niigata University
-
- Togashi Koume
- Department of Agriculture, Faculty of Agriculture, Niigata University
-
- Sugimoto Hayuki
- Graduate School of Science and Technology, Niigata University Department of Agriculture, Faculty of Agriculture, Niigata University
-
- Suzuki Kazushi
- Graduate School of Science and Technology, Niigata University Department of Agriculture, Faculty of Agriculture, Niigata University
抄録
<p>We investigated the presence and functionality of the carbon storage regulator (Csr) system in Aeromonas salmonicida SWSY-1.411. CsrA, an RNA-binding protein, shared 89% amino acid sequence identity with Escherichia coli CsrA. CsrB/C sRNAs exhibited a typical stem-loop structure, with more GGA motifs, which bind CsrA, than E. coli. CsrD had limited sequence identity with E. coli CsrD; however, it contained the conserved GGDEF and EAL domains. Functional analysis in E. coli demonstrated that the Csr system of A. salmonicida influences glycogen biosynthesis, biofilm formation, motility, and stability of both CsrB and CsrC sRNAs. These findings suggest that in A. salmonicida, the Csr system affects phenotypes like its E. coli counterpart. In A. salmonicida, defects in csr homologs affected biofilm formation, motility, and chitinase production. However, glycogen accumulation and protease production were unaffected. The expression of flagellar-related genes and chitinase genes was suppressed in the csrA-deficient A. salmonicida. Northern blot analysis indicated the stabilization of CsrB and CsrC in the csrD-deficient A. salmonicida. Similar to that in E. coli, the Csr system in A. salmonicida comprises the RNA-binding protein CsrA, the sRNAs CsrB and CsrC, and the sRNA decay factor CsrD. This study underscores the conservation and functionality of the Csr system and raises questions about its regulatory targets and mechanisms in A. salmonicida.</p>
収録刊行物
-
- The Journal of General and Applied Microbiology
-
The Journal of General and Applied Microbiology advpub (0), 2024
公益財団法人 応用微生物学・分子細胞生物学研究奨励会