Human Keratinocyte Entry of Noninvasive <i>Streptococcus dysgalactiae</i> Subsp. <i>equisimilis</i> from Humans and Companion Animals: Relatedness with Lancefield Group, Source, Virulence-Associated Genes, and Antimicrobial Resistance Phenotype
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- Yoshida Haruno
- Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Ōmura Satoshi Memorial Institute, Kitasato University, Japan
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- Takayama Yoshiko
- Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Ōmura Satoshi Memorial Institute, Kitasato University, Japan Division of Clinical Laboratory, Byotai-Seiri Laboratory, Japan
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- Goto Mieko
- Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Ōmura Satoshi Memorial Institute, Kitasato University, Japan
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- Maeda Takahiro
- Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Ōmura Satoshi Memorial Institute, Kitasato University, Japan
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- Tsuyuki Yuzo
- Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Ōmura Satoshi Memorial Institute, Kitasato University, Japan Division of Clinical Laboratory, Sanritsu Zelkova Veterinary Laboratory, Japan
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- Takahashi Takashi
- Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Ōmura Satoshi Memorial Institute, Kitasato University, Japan
Bibliographic Information
- Other Title
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- Human Keratinocyte Entry of Noninvasive Streptococcus dysgalactiae Subsp. equisimilis from Humans and Companion Animals : Relatedness with Lancefield Group, Source, Virulence-Associated Genes, and Antimicrobial Resistance Phenotype
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Abstract
<p>We evaluated the cell invasion ability (CIA) of non-invasive Streptococcus dysgalactiae subsp. equisimilis using human keratinocytes and determined the association of CIA populations with their hosts and microbiological traits. Forty-two isolates from humans and companion animals were selected with host information. In addition to CIA, virulence-associated gene (VAG, spegg–ska–scpA–inlA–sicG–brpA–prtF1–prtF2–lmb–cbp–srtp1–srtp2) profiling, emm genotyping, multilocus sequence typing, and antimicrobial resistance (AMR) phenotyping/genotyping were performed. We designated CIA values higher than the mean of all isolates as high-frequency and those lower than the mean as low-frequency. Differences in the CIA between the different sources and Lancefield groups were assessed. We analyzed the association between high- and low-frequency CIA and VAG, emm genotype, sequence type/clonal complex, and AMR phenotype/genotype. Based on the mean (19.368 colony-forming units/100 cells) of 42 isolates, eight isolates had high-frequency CIA, whereas 34 had low-frequency CIA. We found an association between low-frequency CIA population and group G isolates, as well as a link between high-frequency CIA population and group C isolates. We also observed associations between low-frequency CIA population and oral/respiratory tract origin, ska, scpA, and lmb detection, and the AMR phenotype. Our observations suggest potential associations between high-/low-frequency CIA and the group, source, VAG, and AMR phenotypes.</p>
Journal
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 77 (1), 25-33, 2024-01-31
National Institute of Infectious Diseases
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Details 詳細情報について
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- CRID
- 1390017434528883968
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- NII Book ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL BIB ID
- 033307509
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- PubMed
- 37779027
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
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- Abstract License Flag
- Disallowed