Dynamics of the Spontaneous Nucleation of Microtubules

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  • IMAI Hiroshi
    Department of Biological Sciences, Graduate School of Science, Osaka University
  • MUTO Etsuko
    Department of Biological Sciences, Faculty of Science and Engineering, Chuo University School of Advanced Science and Engineering, Waseda University

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Other Title
  • 微小管の自発的核生成
  • ビショウカン ノ ジハツテキカク セイセイ

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Abstract

<p>The structural pathway of spontaneous microtubule nucleation was identified for the first time using recombinant mutant tubulin with a high nucleation rate, rapid flash-negative stain electron microscopy, and kinetic analyses of the size of critical nucleus. The single-stranded tubulin oligomers assembled during the early stage of polymerization had a variety of lengths and curvatures; however, the majority of these oligomers dissociated to tubulin. It was the few straight oligomers exceeding the critical size that underwent lateral interaction, had two-dimensional growth into sheets, and eventually closed into microtubules.</p>

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 63 (6), 299-302, 2023

    The Biophysical Society of Japan General Incorporated Association

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