Dynamics of the Spontaneous Nucleation of Microtubules
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- IMAI Hiroshi
- Department of Biological Sciences, Graduate School of Science, Osaka University
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- MUTO Etsuko
- Department of Biological Sciences, Faculty of Science and Engineering, Chuo University School of Advanced Science and Engineering, Waseda University
Bibliographic Information
- Other Title
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- 微小管の自発的核生成
- ビショウカン ノ ジハツテキカク セイセイ
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Abstract
<p>The structural pathway of spontaneous microtubule nucleation was identified for the first time using recombinant mutant tubulin with a high nucleation rate, rapid flash-negative stain electron microscopy, and kinetic analyses of the size of critical nucleus. The single-stranded tubulin oligomers assembled during the early stage of polymerization had a variety of lengths and curvatures; however, the majority of these oligomers dissociated to tubulin. It was the few straight oligomers exceeding the critical size that underwent lateral interaction, had two-dimensional growth into sheets, and eventually closed into microtubules.</p>
Journal
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- Seibutsu Butsuri
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Seibutsu Butsuri 63 (6), 299-302, 2023
The Biophysical Society of Japan General Incorporated Association
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Details 詳細情報について
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- CRID
- 1390017444754286848
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- NII Book ID
- AN00129693
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- ISSN
- 13474219
- 05824052
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- NDL BIB ID
- 033231751
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
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- Abstract License Flag
- Disallowed