Time-dependent changes in retinoids content in liver and adipose tissue after feeding of a vitamin A-deficient diet to mice

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  • KATO-SUZUKI Mira
    Laboratory of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University
  • OKAMATSU-OGURA Yuko
    Laboratory of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University
  • INANAMI Osamu
    Laboratory of Radiation Biology, Faculty of Veterinary Medicine, Hokkaido University
  • KIMURA Kazuhiro
    Laboratory of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University

抄録

<p>Vitamin A is an important nutrient for multiple physiological functions. To elucidate the role of vitamin A in vivo, vitamin A-deficient diets have been often used in mice to establish a vitamin A-deficiency model. However, the information on the appropriate feeding periods and time course of changes in vitamin A content in organs after the start of vitamin A-deficient diet feeding is lacking. This study aimed to assess the retinoids levels in liver and white adipose tissue in mice fed a vitamin A-deficient diet for £8 weeks. High-performance liquid chromatography was used to measure the retinoids levels in liver and white adipose tissue every 2 weeks for £8 weeks. Vitamin A-deficient diet feeding significantly decreased retinol in the liver over 6 weeks, but retinyl palmitate, a main storage form of vitamin A, was not changed over 8 weeks. The plasma retinol level remained constant throughout the experiment. In white adipose tissue, retinyl palmitate gradually decreased over 8 weeks. These results indicate that vitamin A-deficient diet feeding longer than 6 weeks reduced retinol in liver and retinyl palmitate in white adipose tissue over 8 weeks, although it is not enough for the induction of a whole-body vitamin A deficiency.</p>

収録刊行物

  • Experimental Animals

    Experimental Animals advpub (0), 2024

    公益社団法人 日本実験動物学会

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