Histological Properties of a Chemically Fixed Human Embryo Visualized with Quantitative Susceptibility Mapping
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- Shirai Toru
- Institute of Pure and Applied Physics, University of Tsukuba, Tsukuba, Ibaraki, Japan
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- Terada Yasuhiko
- Institute of Pure and Applied Physics, University of Tsukuba, Tsukuba, Ibaraki, Japan
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- Kose Katsumi
- MRI simulations Inc, Tokyo, Japan
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- Yamada Shigehito
- Congenital Anomaly Research Center, Kyoto University Graduate School of Medicine, Kyoto, Kyoto, Japan
抄録
<p>A chemically fixed Carnegie stage 23 (approximately 56 days of gestation) human embryo specimen was imaged using 3D spin-echo and gradient-echo sequences in a static magnetic field strength of 4.74T, and a quantitative susceptibility map was calculated using the 3D gradient-echo image. The acquired 3D microscopic images (90 μm cube voxel size) clarified the relationship between R2 (transverse relaxation rate), R2* (apparent transverse relaxation rate), and magnetic susceptibility in the heart, liver, kidney, and spinal cord. The results suggested that the R2* and magnetic susceptibility in each tissue were probably due to paramagnetic iron ions originating from erythrocytes. The large R2* (~130 s-1) and magnetic susceptibility (~0.122 ppm) in the liver were attributed to its hemopoietic function. A large magnetic susceptibility (~0.116 ppm) was also observed in the spinal cord, but we conclude that more detailed future studies are needed.</p>
収録刊行物
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- Magnetic Resonance in Medical Sciences
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Magnetic Resonance in Medical Sciences advpub (0), 2024
日本磁気共鳴医学会