Cycloheximide in the nanomolar range inhibits seed germination of <i>Orobanche minor</i>

DOI Web Site 参考文献41件
  • Nogami Ryosuke
    Graduate School of Agriculture, Osaka Metropolitan University
  • Nagata Mari
    Graduate School of Life and Environmental Sciences, Osaka Prefecture University
  • Imada Risa
    Graduate School of Life and Environmental Sciences, Osaka Prefecture University
  • Kai Kenji
    Graduate School of Agriculture, Osaka Metropolitan University Graduate School of Life and Environmental Sciences, Osaka Prefecture University
  • Kawaguchi Takashi
    Graduate School of Agriculture, Osaka Metropolitan University Graduate School of Life and Environmental Sciences, Osaka Prefecture University
  • Tani Shuji
    Graduate School of Agriculture, Osaka Metropolitan University Graduate School of Life and Environmental Sciences, Osaka Prefecture University

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<p>From the 992 samples of culture extracts of microorganisms isolated from soil in Japan, we found that the extract of Streptomyces sp. no. 226 inhibited Orobanche minor seed germination without significantly affecting the seed germination of Trifolium pratense and the growth of Aspergillus oryzae and Escherichia coli. Using ESI-MS, 1H-NMR, and 13C-NMR, we identified the active compound as cycloheximide. Cycloheximide had half-maximum inhibitory concentrations of 2.6 ng/mL for the inhibition of seed germination of O. minor and 2.5 µg/mL for that of the conidial germination of A. oryzae. Since cycloheximide is known to inhibit translation by interacting with ribosomal protein L28 (RPL28) in yeast, we investigated whether RPL protein of O. minor plays a critical role in the inhibition of O. minor seed germination. Our data suggested that O. minor RPL27A was not sensitive to cycloheximide by comparing it to the strain expressing S. cerevisiae RPL28. These findings suggest the presence of an unidentified mechanism by which cycloheximide hinders O. minor seed germination.</p>

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