Acclimatization of Endangered <i>Morus boninensis</i> Grown <i>in vitro</i> Using Re-purposed Plastic Drink Bottles

DOI Web Site 9 References
  • Endoh Keita
    Forest Tree Breeding Center, Forestry and Forest Products Research Institute
  • Oribe Yuichiro
    Forest Tree Breeding Center, Forestry and Forest Products Research Institute
  • Kitayama Tomohiro
    Ogasawara Subtropical Agricultural Research Center, Tokyo Metropolitan Government Ogasawara Islands Branch Office
  • Murata Souma
    Ogasawara Subtropical Agricultural Research Center, Tokyo Metropolitan Government Ogasawara Islands Branch Office
  • Yoshii Yoshiko
    Non-profit Organization for Research of Wild Organisms in Ogasawara
  • Yokoya Midori
    Non-profit Organization for Research of Wild Organisms in Ogasawara
  • Hanaoka So
    Faculty of Agriculture, Shizuoka University
  • Matsushita Michinari
    Forest Tree Breeding Center, Forestry and Forest Products Research Institute
  • Hasebe Tatsutaka
    Forest Tree Breeding Center, Forestry and Forest Products Research Institute
  • Tamaki Satoshi
    Forest Tree Breeding Center, Forestry and Forest Products Research Institute
  • Kuramoto Noritsugu
    Forest Tree Breeding Center, Forestry and Forest Products Research Institute
  • Yamada Hiroo
    Forest Tree Breeding Center, Forestry and Forest Products Research Institute

Bibliographic Information

Other Title
  • 飲料用ペットボトルを再利用した絶滅危惧種オガサワラグワの培養苗の馴化

Abstract

<p>Morus boninensis is a critically endangered tree endemic to Japan's Ogasawara Islands. As part of ongoing ex situ conservation efforts, we have been raising M. boninensis seedlings in vitro to supplement wild populations on the Ogasawara Islands. However, local facilities are insufficient to maintain trees in vitro, and many seedlings die while acclimating to the potted environment. Thus, an improved approach to acclimatization is needed. In this study, we examined a method of acclimating M. boninensis seedlings grown in vitro using re-purposed plastic beverage bottles (PB method). The plastic bottles were cut into two pieces about 7 cm from the bottom. The upper portion was used as a cover and the lower portion as a saucer. After transplanting an in vitro-grown M. boninensis specimen into a pot, water was added to the medium until the saucer was full. The cover was placed over the specimen, which was then acclimated as normal. This approach yielded a 66.7-100％ survival rate. Many clonally propagated seedlings have been produced on the Ogasawara Islands; the PB method shows promise for improving their survival and aiding the conservation of a critically endangered species.</p>

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