Mechanism of macrophage injury by amorphous silica nanoparticles
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- YAMAZAKI Kyoka
- Department of Occupational and Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- VRANIC Sandra
- Department of Occupational and Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine
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- WATANABE Eri
- Department of Occupational and Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- MIYAKAWA Kayoko
- Department of Occupational and Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- TAKEUCHI Sakie
- Department of Occupational and Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- OSADA Yurika
- Department of Occupational and Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- ICHIHARA Sahoko
- Department of Enviornmental and Preventive Medicine, Jichi Medical University School of Medicine
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- WU Wenting
- Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine
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- ZONG Cai
- Department of Occupational and Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine
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- SAKURAI Toshihiro
- Department of Occupational and Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- SATO Akira
- Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- HARA Yasushi
- Research Institute of Biomedical Sciences, Tokyo University of Science
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- TERASHIMA Yuya
- Research Institute of Biomedical Sciences, Tokyo University of Science
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- MATSUSHIMA Kouji
- Research Institute of Biomedical Sciences, Tokyo University of Science
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- SUZUKI Toshihiro
- Research Institute of Biomedical Sciences, Tokyo University of Science
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- ABE Ryo
- Research Institute of Biomedical Sciences, Tokyo University of Science
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- BOLAND Sonja
- University of Paris VII Diderot, Paris, France
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- TRAN Lang
- Institute of Occupational Medicine, Edinburgh, UK
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- ICHIHARA Gaku
- Department of Occupational and Environmental Health, Faculty of Pharmaceutical Sciences, Tokyo University of Science Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine
Bibliographic Information
- Other Title
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- 非晶質シリカナノ粒子によるマクロファージ傷害メカニズム
Abstract
<p>Introduction: Recent advances in nanotechnology have led to the development and use of nanomaterials. Despite concerns about human exposure to nanomaterials in various environments, their safety evaluation remains elusive. Amorphous silica nanoparticles (SiO2NPs) are a particularly widely used nanomaterial, but our laboratory has previously reported that bare SiO2NPs with hydroxyl groups (OH-SiO2NPs) may be more injurious to macrophages in vitro and in vivo than SiO2NPs modified with amino or carboxyl groups. This study aimed to investigate the mechanism of cytotoxicity by OH-SiO2NPs in vitro. </p><p>Methods: We exposed the mouse macrophage cell line RAW264.7 to OH-SiO2NPs and treated it with inhibitors of various programmed cell death pathways to examine the relationship between cytotoxicity and each cell death pathway. In addition, mRNA expression levels of inflammatory chemokines and cytokines were measured by RT-qPCR. </p><p>Results and Discussion: Co-treatment with apoptosis and necroptosis inhibitors improved the cell viability, although sole treatment with apoptosis or necroptosis inhibitor did not. The results with inhibitors of various cell death pathways suggest that apoptosis or necroptosis is partially involved in the mechanism of cell death. Additionally, exposure to SiO2NPs was found to induce the production of pro-inflammatory cytokines and chemokines by macrophages, however, the relationship between this inflammatory response and cytotoxicity should be further investigated.</p>
Journal
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- Annual Meeting of the Japanese Society of Toxicology
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Annual Meeting of the Japanese Society of Toxicology 50.1 (0), P1-067S-, 2023
The Japanese Society of Toxicology
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Details 詳細情報について
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- CRID
- 1390017920607824768
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- Text Lang
- ja
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- Data Source
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- JaLC
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- Abstract License Flag
- Disallowed