Whole Genome Analysis of Two Pectobacterium Species Isolated from Blackleg Disease of Potato Occurred in Nagasaki Prefecture, Japan

DOI IR (HANDLE) Open Access
  • Man Quang LE
    Laboratory of Plant Pathology, Division of Agrobiological Sciences, Department of Bioresource Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University Laboratory of Plant Disease Diagnostic, Division of Pest and Natural Enemy Identification, Plant Protection Research Institute, Vietnam Academy of Agricultural Science
  • IIYAMA Kazuhiro
    Laboratory of Plant Pathology, Division of Agrobiological Sciences, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University
  • NISHIYAMA Haruki
    Laboratory of Plant Pathology, Division of Agrobiological Sciences, Department of Bioresource Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • OTOFUJI Hana
    Laboratory of Plant Pathology, Division of Agrobiological Sciences, Department of Bioresource Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • SUGA Yasuhiro
    Nagasaki Agricultural and Forestry Technical Development Center
  • TSUCHIYA Kenichi
    Laboratory of Plant Pathology, Division of Agrobiological Science, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University
  • FURUYA Naruto
    Laboratory of Plant Pathology, Division of Agrobiological Science, Department of Bioresource Sciences, Faculty of Agriculture, Kyushu University

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Description

In Japan, Dickeya dianthicola, D. chrysanthemi, Pectobacterium atrosepticum, P. parmentieri, and P. brasiliense are known as blackleg disease pathogens of potato. A transient outbreak of the blackleg disease occurred in Nagasaki Prefecture, and the pathogens were identified as P. parmentieri and P. brasiliense. Because repetitive extragenic palindromic PCR showed clonal relatedness among strains in each species, two Nagasaki strains (NK5 and NK14) were selected as the representative strains, and their whole genome sequences were determined. Average nucleotide identity analysis supported the identification in the previous study. Multilocus sequence analysis with seven housekeeping genes indicated the relationship between NK14 and other strains isolated in Japan, Russia, China, Switzerland, Poland, and Belgium. NK5 was related to the strains from various geographic locations including Syria, China, Canada, South Korea, Netherlands, Algeria, South Africa, Mexico, and Belarus. Subsequently, core genome multilocus sequence typing was performed with publicly available sequences, indicating that NK14 and NK5 were most closely related to the strains Poland/Russian and Netherlands/Belarus/Russia, respectively. This study concerns the first phylogenetic analyses based on the whole genome sequences of Japanese strains of P. parmentieri and P. brasiliense, and contributes to the molecular epidemiological analysis of the pathogen of potato blackleg.

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Details 詳細情報について

  • CRID
    1390017965044081920
  • NII Book ID
    AA00247166
  • DOI
    10.5109/7169358
  • HANDLE
    2324/7169358
  • ISSN
    00236152
  • Text Lang
    en
  • Article Type
    departmental bulletin paper
  • Data Source
    • JaLC
    • IRDB
    • Crossref
    • OpenAIRE

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