Characterizing Bacterial Communities among Healthy, Peri-implant Mucositis, and Peri-implantitis Statuses by 16S rRNA Gene Amplicon Sequencing

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  • Takahiko NAGAI
    Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
  • Takahiko SHIBA
    Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine
  • Keiji KOMATSU
    Department of Lifetime Oral Health Care Science, Tokyo Medical and Dental University
  • Shunsuke MATSUMURA
    Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
  • Tatsuro KOYANAGI
    Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
  • Takashi NEMOTO
    Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
  • Ryota KOBAYASHI
    Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
  • Yasuo TAKEUCHI
    Department of Lifetime Oral Health Care Science, Tokyo Medical and Dental University
  • Takanori IWATA
    Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

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Description

<p>  Introduction : Dental implants are crucial for treating missing teeth, while a dysbiotic microbiome often causes biological implant complications leading to implant loss.</p><p>  Materials and Methods : To clarify the bacterial community profiles of a healthy implant (HI), peri-implant mucositis (MI), and peri-implantitis (PI), 25 subgingival plaques each were collected and analyzed by 16S rRNA gene amplicon sequencing.</p><p>  Results : Bacterial diversity at the MI and PI sites was higher than that at the HI site, while no difference was observed between MI and PI sites. Desulfomicrobium and Saccharibacteria (TM7) [G-1] bacterium HMT 349 showed a significantly higher abundance at the MI site than at the HI site, suggesting that these bacterial taxa might be associated with the induction of peri-implant inflammation. Well-known disease-associated bacteria such as Porphyromonas gingivalis, Treponema, and Fusobacterium nucleatum subsp. vincentii exhibited significantly higher abundance at the PI site compared to the HI site. Furthermore, Capnocytophaga granulosa and Prevotella sp. HMT 304 were more abundant at the PI site than at the MI site.</p><p>  Conclusion : These bacteria appeared to be involved in the pathogenic state of dental implants, especially peri-implantitis. Our study highlights the microbial intricacies in healthy implants and peri-implant diseases, providing insights for diagnostic and therapeutic strategies.</p>

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