Comparison of enrichment media for recovery of <i>Salmonella enterica</i> after storage at various temperatures and alkaline stress exposure
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- Sakazume Noriko
- Hokkaido Kushiro Livestock Hygiene Service Center
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- Hara Kiwako
- Hokkaido Kushiro Livestock Hygiene Service Center
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- Fujihara Masatoshi
- Hokkaido Kushiro Livestock Hygiene Service Center
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説明
Rectal swabs containing Salmonella enterica may be exposed to desiccation stress while awaiting laboratory isolation. Growth of injured S. enterica is inhibited on selective media but the extent to which this varies across different media remains unclear. After storage at 25 °C, we compared growth recovery using four enrichment media and revealed that log 2.7 CFU and > log 4.0 CFU was required to confirm the growth of monophasic S. enterica Typhimurium K13-4-49 in buffered peptone water and selective enrichment media (Hajna tetrathionate broth, tetrathionate broth, and modified semisolid Rappaport agar for stab culture), respectively. Since < log 1.7 CFU was required for all four media, we recommend sample storage at ≤ 4 °C or the application of transport medium.
収録刊行物
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- Japanese Journal of Veterinary Research
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Japanese Journal of Veterinary Research 72 (1), 36-40, 2024-06-28
JJVR編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390019158920101120
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- NII書誌ID
- AA1202891X
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- ISSN
- 2758447X
- 00471917
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- HANDLE
- 2115/92690
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
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- 抄録ライセンスフラグ
- 使用不可