Exploring novel oxidized phospholipids to elucidate an exhaustive understanding of their functions

<p>Lipid peroxidation generates numerous oxidized lipids and has shown its essential role in cell biology and human health, in which oxidized phospholipids (OxPLs) are the crucial lipids reportedly involved in the pathogenesis and progression of numerous diseases. Although OxPLs are known to modulate various biological functions, their precise role remains an ongoing debate due to the lack of structural information and dedicated analytical methods. In this study, we used mass spectrometry and HPLC techniques to explore and isolate novel types of OxPLs, such as oxygenated and truncated acyl chains of phospholipids, and then purifying by preparative HPLC. It has been found that the production of oxidized lipids varies based on the oxidation methods. Additionally, we discovered that novel OxPLs identified in this study indicate multiple activities relating to inflammation and cell signaling. The modification of phospholipids by oxidation is one of the critical determinants of its interactions with other molecules, proteins, and the lipid bilayer, influencing membrane properties and activities. Developing the OxPLs library using mass spectrometry and OxPLs purifying are expected to provide a comprehensive understanding of OxPLs involved in diseases and pave the way for proposing candidate compounds for innovative therapeutic strategies in lipid peroxidation-related diseases.</p>