Rapid determination of theophylline and antiepiletic drugs in serum by column-switching HPLC.

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  • CHIBA Ryoko
    Showa of Pharmaceutical University,3-3165,Higashitamagawagakuen,machida-shi,Tokyo 194-8543
  • YAMAZAKI Hiroyuki
    Scientific Instrument Division,Tosoh Co.,2743-1,Hayakawa,Ayase-shi,Kanagawa 252-1123
  • UMINO Masuo
    Scientific Instrument Division,Tosoh Co.,2743-1,Hayakawa,Ayase-shi,Kanagawa 252-1123
  • URAYAMA Isao
    Toho University School of Medicine,6-11-1,Omorinishi,Ota-ku,Tokyo 143-8541
  • TANAKA Akira
    Showa of Pharmaceutical University,3-3165,Higashitamagawagakuen,machida-shi,Tokyo 194-8543

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Other Title
  • カラムスイッチング高速液体クロマトグラフィーによる血清中のテオフィリン及び抗てんかん薬の迅速定量
  • カラムスイッチング コウソク エキタイ クロマトグラフィー ニ ヨル ケッセイ チュウ ノ テオフィリン オヨビ コウテンカンヤク ノ ジンソク テイリョウ

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Abstract

The rapid determination of theophylline and antiepileptic drugs(phenobarbital, carbamazepine, phenytoin and valproic acid) in human serum using a combination of a short pretreatment column(TSKprecolumn BSA-ODS/S) and a short analytical column(TSKgel Super-ODS) was investigated.Under the optimized analytical conditions, theophylline and four antiepileptic drugs were eluted within 14min.The relationship between the sample amount and the peak areas of five drugs indicated good linearity over the range from 1.0 to 500mg/l(valproic acid) and 0.2 to 110mg/l(other drugs), respectively.Five drugs from spiked serum were recovered quantitatively.The repeatability and reproducibility of the peak areas of standard and spiked serum were less than 1.6%.The drug concentrations in human serum determined by a enzyme immunoassay method and a column-switching method showed good agreement.Therefore, this column-switching method is very suitable for therapeutic drug monitoring(TDM) in biological fluids.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 49 (3), 195-199, 2000

    The Japan Society for Analytical Chemistry

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