Special Articles: Environmental Sciences and Analytical Chemistry. Simple in situ determination of dissolved oxygen in water using Methylene Blue.

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  • 特集　環境科学と分析化学　メチレンブルー発色法を用いた水中溶存酸素の簡易現場定量法
  • メチレン ブルー ハッショクホウ オ モチイタ スイチュウ ヨウゾン サンソ
  • メチレンブルー発色法を用いた水中溶存酸素の簡易現場定量法(<特集>環境科学と分析化学)

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Abstract

A simple visual method has been proposed for the in situ determination of dissolved oxygen in environmental waters, based on spectrophotometric studies with Methylene Blue. The reduced Methylene Blue was prepared in a graduated syringe by the reduction with glucose in aqueous-ethanol solution containing sodium hydroxide. Then, the sample was introduced into the syringe. The oxidation of the reduced Methylene Blue with the dissolved oxygen was completed almost instantaneous, the color changing from colorless to blue. The color intensity was proportional to the concentration of dissolved oxygen. The color remained stable for 90 min. Up to 13 μg/ml dissolved oxygen can be determined visually and also spectrophtometrically at 600 nm with a relative standard deviation of 3.9%. The visual method can be applied to in situ determination of dissolved oxygen in seawater and brackish water as well as fresh water.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 43 (12), 1059-1063, 1994

    The Japan Society for Analytical Chemistry

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