Special Articles: Separation. Part 2. Chromatography and Capillary Electrophoresis. Development of 3-(N-substituted)aminopropylsilyl silica gels having .PI.-electron systems and a preliminary research of their application to HPLC.

AKIYAMA Shuzo, NAKASHIMA Kenichiro, INOUE Keiko, KURODA Naotaka, NAKAZUMI Hiroyuki

doi:10.2116/bunsekikagaku.42.12_817

Bibliographic Information

Other Title

特集：　分離　（その２）　クロマトグラフ・キャピラリー電気泳動分離　π電子系を有する３‐（Ｎ‐置換）アミノプロピルシリルシリカゲルの開発と高速液体クロマトグラフィーへの利用に関する基礎的研究
パイデンシケイオユウスル 3 N チカンアミノプロピルシリルシリカゲ
π電子系を有する3-(N-置換)アミノプロピルシリルシリカゲルの開発と高速液体クロマトグラフィーへの利用に関する基礎的研究<特集>分離(その2

Search this article

Abstract

A series of 3-(N-substituted)aminopropylsilyl silica gels newly developed for HPLC has been reported for an easy separation of biologically active compounds, such as water-soluble vitamins, by isocratic elution. Through expectations of a π-π interaction, five kinds of 3-aminopropylsilyl silica gel were prepared; these were substituted with π-π electron systems at the amino group by the reaction of the gel with two species of organic dyes (5-amino-2, 3-dicyano-1, 4-naphthoquinone or 1, 4-diamino-9, 10-anthraquinone-2, 3-dicarboxylic anhydride) or aromatic dicarboxylic anhydrides (phthalic anhydride, 2, 3-naphthalenic anhydride or 1, 8-naphthalenic anhydride). Basic examinations of the characteristics of the HPLC columns prepared by using these gels were carried out in order to separate compounds related to nucleic acids, i.e. adenine derivatives. The preparation of gels immobilized with organic dyes is new, and its use for HPLC is hopefully expected for separation by a π-π interaction mode.

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 42 (12), 817-823, 1993

The Japan Society for Analytical Chemistry

Keywords

Details 詳細情報について

Export