Rapid determination of organic solvent metabolites in urineby reversed phase HPLC.

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  • 逆相高速液体クロマトグラフィーによる尿中有機溶剤代謝物の迅速定量
  • ギャクソウ コウソク エキタイ クロマトグラフィー ニ ヨル ニョウチュウ ユ

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Abstract

We have developed an improved method for the determination of organic solvent metabolites(HAs), hippuric acid(HA), mandelic acid(MA), ο-methylhippuric acid(ο-MHA), m-methylhippuric acid (m-MHA), p-methylhippuric acid(p-MHA) and creatinine(CR), in urine by reversed phase HPLC. The separation was carried out with a stainless-steel column (4.0mm×200mm) packed with Wakosil II-5C18-100 and 8% 2-propano1/10mM phosphate buffer (pH2.4) containing 6mM 1-octanesulfonic acid sodium salt as a mobile phase (1.0ml/min). A UV monitor (210 nm) was used for monitoring. Reproducibility of this method was excellent. Calibration plots between 10 and 1000ng/injection were linear and passed through the origin of the coordinate system. This method was used for the determination of HAs in urine on volunteers.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 40 (12), 895-898, 1991

    The Japan Society for Analytical Chemistry

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