Analysis of alcohols by superheated water chromatography with flame ionnization detection

NAKAJIMA Ryoji, YARITA Takashi, SHIBUKAWA Masami

doi:10.2116/bunsekikagaku.52.305

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超高温水クロマトグラフィー‐水素炎イオン化検出法によるアルコール類の分析
超高温水クロマトグラフィー-水素炎イオン化検出法によるアルコール類の分析
チョウコウオンスイクロマトグラフィースイソエンイオンカケンシュツホウニヨルアルコールルイノブンセキ

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Abstract

A method for the superheated water chromatography (SWC)-flame ionization detection (FID) of alcohols was developed. An FID system was connected to an SWC system by splitting the mobile phase of the SWC system and then introducing it into the FID system via a fused-silica capillary tube. The FID conditions (the position of the end of the capillary tube in the FID system, flow rate of hydrogen gas, and flow rate of helium makeup gas) were optimized so as to obtain a stable and sensitive detection of alcohols; the detection limits (S/N=3) of methanol, ethanol, 1-propanol, 1-butanol, and 1-pentanol were in the range of 26∼57 ng (as injected amounts into the SWC system) under the optimized conditions. The retention of alcohols in SWC decreased with an elevation in the column temperature as well as a decrease in the mobile-phase pressure. The retention mechanism was characterized using a thermodynamic theory that has been used for describing retention in conventional high-performance liquid chromatography. The application of the present method to determination of ethanol in wine samples was also demonstrated, in which the observed values were comparable to those obtained by gas chromatography/mass spectrometry.<br>

Journal

BUNSEKI KAGAKU

BUNSEKI KAGAKU 52 (5), 305-309, 2003

The Japan Society for Analytical Chemistry

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